Use of the CRISPR (clustered regularly interspaced short palindromic repeats) and associated Cas9 enzyme for genome editing has been a major technological breakthrough, making genome modification in cells or organisms faster, more efficient, and more robust than previous genome editing methods. The Alt-R CRISPR-Cas9 System is an optimized genome editing solution for producing on-target, double-stranded DNA breaks.
We have also developed an alternative Alt-R CRISPR-Cas12a (Cpf1) System to open up CRISPR editing to additional areas in genomes.
|Cas9 system||Cas12a system|
|Applications||General genome editing|
|Cas9 crRNA:tracrRNA (option 1)|
|Cas9 sgRNA (option 2)||—|
|Current recommendations for Alt-R RNP delivery|
* Molecular weight of Alt-R nuclease
† N = any base; V = A, C, or G
IDT does not sell gene therapy kits and nothing sold by IDT should be construed as a gene therapy kit. Customers should not use any IDT products for self-administration.