The rhAmpSeq CRISPR Analysis System allows quick and accurate quantification of CRISPR-Cas edits. Our proprietary RNase H2-dependent PCR technology generates amplicon libraries for targeted sequencing on Illumina® NGS platforms. The system also includes
an advanced but accessible cloud-based data analysis pipeline for quantification of on- and off-target edits.
- Best-in-class insertion/deletion (indel) quantification of genome editing
- Batch analysis of your results (multiplex up to 500 targets)
- High sequencing coverage uniformity and percentage of mapped reads
- Intuitive data analysis that requires no bioinformatics expertise
- Sample-to-analysis in under a week
Harnessing the power of rhAmp™ PCR for characterizing on- and off-target editing
The rhAmpSeq CRISPR Analysis System depends on our proprietary rhAmp PCR technology
. Using RNA-base–containing blocked primers (rhAmp primers), this technology harnesses the intrinsic specificity of the RNase H2 enzyme to cleave DNA:RNA duplexes. Due to its inherent mitigation of primer dimers, rhAmp PCR exhibits high specificity, even in multiplex reactions. The rhAmpSeq system combines this innate advantage with a convenient workflow that requires only two PCR steps (Figure 1) to generate amplicon libraries for Illumina sequencing platforms. Figure 1 shows both amplification steps in the rhAmpSeq workflow, highlighting the role of rhAmp PCR technology during the first step (Targeted rhAmp PCR 1). Coupled with our industry-leading oligo manufacturing process, the rhAmpSeq system offers fast and cost-effective analysis of your CRISPR results.